Offline and Online IP-LC-MS/MS for Targeted Quantitation of ADCs and Transgenic Biomarkers

讲座信息

时间：2024年12月06日（周五）09:00-10:00

地点：卫光生命科学城4层401-E会议室

主讲人：Jianhui Zhu,PhD

主持人：殷海娣 多组学检测分析平台主管

腾讯会议：247 456 983

主讲人

Jianhui Zhu,PhD

Director of Bioanalytical Sciences, 

NovaBioAssays LLC, Boston

Dr. Zhu is currently Director of Bioanalytical at NovaBioAssays LLC, a CRO based in Boston offering innovative LC-MS and LC-MS/MS solutions for characterization and quantitative analysis of therapeutic proteins,antibodies,small molecules, and oligonucleotides in support of drug development for biotech and pharmaceutical industries. Prior to NovaBioAssays, Dr. Zhu joined Dr. David M. Lubman’s group as a postdoc and later served as Lead Research Scientist in the Laboratory of Cancer Proteomics in the Department of Surgery at the University of Michigan. She led portfolio strategy in mass spec-based cancer proteomics for biomarker discovery and validation, including protein glycosylation, for early cancer detection. With 15+ years of in-depth experience in developing LC-MS/MS and immunoprecipitation (IP) methods, her expertise lies in protein characterization and quantitation in biological matrices for DMPK and PK/PD studies. Dr. Zhu has published 57 peer-reviewed research articles and received NCI Research Specialist Award in 2018. Dr. Zhu earned her PhD in Bioinorganic Chemistry in 2009 underthe direction of Professor Zijian Guo at Nanjing University, School of Chemistry and Chemical Engineering.

报告摘要

Offline IP-LC-MS/MS quantitation

1.Capture antibody screening

2.Conjugation of biotinylated antibody to streptavidin beads

3.MRM transition and CE optimization

4.FDA Bioanalytical Method Validation Guidance for Industry

Online IP-LC-MS/MS quantitation

1.Peptide level IP

2.Custom Anti-id antibody

3.LC method setup (loading, trap, and separation)

4.Data interpretation

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