string(16) "en/equipment/715" Analytical Ultracentrifuge-Shenzhen Medical Academy of Research and Translation
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Technical Parameters | Sample Submission Requirements | Application Areas
Analytical Ultracentrifuge

Analytical Ultracentrifuge

Brand:

Beckman

Model:

Optima AUC

Affiliated Platform:

Sample Preparation and Analysis Core Facility

Placement:

Weiguang Life Science Park 1A Building 308F

Contact Person:

Xie Xingqiao, Zhang Jing

Phone:

13671959351，15901507229

Email:
Instrument Reservation
Technical Parameters Sample Submission Requirements Application Areas

1. Separation system

1.1 The maximum speed of the main engine is 60,000 rpm, and the speed accuracy is 20 rpm

1.2 The temperature setting range is 4-40 °C, and the accuracy is 1 °C

1.3 Number of samples separated (simultaneous experiments): 21

1.4 Cell selection: 2/6/8 wells

1.5 rotor selection: 4-well cell analysis rotor at 60,000 rpm; 8-well cell analysis rotor up to 50,000 rpm.

1.6 Minimum injection volume: 0.025ml

1.7 Vacuum: 5 Pa

1.8 Setting time: up to 999 hours and 59 minutes or continuous operation

2. Detection system

2.1 There are two detection systems of ultraviolet light and interference light, and the detector can be operated independently and simultaneously

2.2 Ultraviolet light detector: detection range 190 – 800 nm, 0-3A absorption, scanning mode divided into radius scanning mode and wavelength scanning mode

2.3 Configure an interferometric light detector

2.4 The device has a diffraction grating

2.5 Automatic detection of baseline correction

2.6 Molecular weight detection range: 10²–10⁸Da

2.7 Sample concentration detection range: 5μg/ml – 2mg/ml (ultraviolet light), 25μg/ml – 5mg/ml (interference light)

2.8 Balance coefficient detection range: 10^-3–10^-8 m

3. Control system

3.1 Computer software control, automatic detection data collection and analysis

3.2 Two experimental analysis methods can be carried out: sedimentation rate and settlement equilibrium

3.3 A variety of data analysis methods for sedimentation rate and sedimentation equilibrium experiments can be provided

3.4 Automatically calculate the sedimentation coefficient of the sample

3.5 Automatically calculate the molecular weight, diffusion coefficient, molecular bonding/separation coefficient of the sample, etc

Interactions between proteins, nucleic acids, sugars, and other molecules; Binding / dissociation constant determination; Absolute molecular weight and sedimentation coefficient determination; determination of the shape, size and buoyancy density of molecules; Determination of protein sample purity, other thermodynamic and kinetic parameters, detection of empty shell rate of viral vector assembly, etc.

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