string(16) "en/equipment/763" Live-cell Label-free Superesolution Confocal Microscope-Shenzhen Medical Academy of Research and Translation
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Technical Parameters | Sample Submission Requirements | Application Areas
Live-cell Label-free Superesolution Confocal Microscope

Live-cell Label-free Superesolution Confocal Microscope

Brand:

Nikon

Model:

AXR-NSPARC/MH-SpotyView

Affiliated Platform:

Biomedical Imaging Core Facility

Placement:

Building 1A, Weigang Life Science Park, 2nd Floor, Room 221

Contact Person:

Yaowen Zhang

Phone:

18390936582

Email:
Instrument Reservation
Technical Parameters Sample Submission Requirements Application Areas

1.Solid-state lasers:

(405/455/488/514/561/640 nm).

2.Objectives:

2x apochromatic objective (N.A. 0.10, W.D. 8.5 mm)

4x apochromatic objective (N.A. 0.20, W.D. 20.0 mm)

10x apochromatic objective (N.A. 0.45, W.D. 4.0 mm)

20x apochromatic objective (N.A. 0.80, W.D. 0.8 mm)

40x apochromatic objective (N.A. 0.95, W.D. 0.21 mm)

60x apochromatic oil immersion objective (N.A. 1.42, W.D. 0.15 mm)

100x apochromatic oil immersion objective (N.A. 1.45, W.D. 0.13 mm)

20x extra-long working distance objective (N.A. 0.45, W.D. 8.2-6.9 mm)

40x extra-long working distance objective (N.A. 0.60, W.D. 3.6-2.8 mm)

40x full-wavelength silicone oil immersion objective (N.A. 1.25, W.D. 0.30 mm)

3.Label-free module:

Label-free imaging resolution: 130 nm in XY direction; 350 nm in Z direction. Maximum field of view for single-shot label-free imaging without layer scanning: 245 μm × 245 μm. Temporal resolution: single 3D detection volume (245 μm × 245 μm × 40 μm) at 1.8 FPS; single 3D detection volume (60 μm × 60 μm × 40 μm) at 8 FPS.

4.Detectors:

Four GaAsP high-sensitivity detectors, with linear variable filters and spectral splitting; and one MA transmitted light detector. NSPARC detector: Nikon's advanced spatial array detector with a maximum resolution of 8192 × 8192; 512 × 512 pixels at 30 fps; 1024 × 1024 pixels at 7.5 fps, with a large field of view (FOV) up to 25 mm.

5.Laser confocal scanning head:

Galvanometer scanner: For high-resolution imaging, scan resolution of 8192 × 8192 pixels, scan speed: fast mode at 10 fps (512 × 512 pixels, bidirectional); 240 fps (512 × 16 pixels, bidirectional).

Resonant scanner: For high-speed imaging, maximum scan resolution of 2048 × 2048 pixels at 7.5 fps. Scan speeds: 2048 × 512 pixels at 30 fps, 2048 × 1024 pixels at 15 fps.

6.Super-resolution detector resolution: 100 nm in XY direction, 300 nm in Z direction.

7.Fluorescence light source: LED fluorescence light source, equipped with four LED sources (385 nm, 475 nm, 550 nm, and 621 nm) and red, green, blue fluorescence excitation blocks.

8.Fully motorized inverted microscope, with a side port field of view of 25 mm.

9.Equipped with a live cell incubator.


1.Nikon's advanced NSPARC spatial array detector offers super-resolution confocal imaging capabilities, enabling high spatial resolution imaging of tissue sections and live cells with fluorescent labels, and delivers clear and sharp multi-layer Z-plane structures. It also achieves high signal-to-noise ratio imaging for 3D observation of thick samples, such as brain nerves, organoids, and organ chips.


2.The label-free module uses 360-degree laser rotational scanning to obtain quantitative three-dimensional physical refractive index (RI) imaging inside cells. It enables rapid super-resolution acquisition of 3D internal structures of cells without labeling or damaging the sample, with ultra-low phototoxicity and no photobleaching. It can clearly visualize subcellular structures or functional regions within cells, such as mitochondria, lipid droplets, nuclear membranes, nucleoli, and cell membranes. The system supports high-throughput, high-content imaging and is compatible with confocal dishes, 12-well plates, 24-well plates, and 96-well plates.


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