Conventional Cell Analyzer BD LSRFortessa X-20
BD
LSRFortessa X-20
Flow Cytometry Core Facility
206, 2nd Floor, Building 1A, Weiguang Life Science Park
chenxinyi, Liujie
18718204132;13760450981
chenxinyi@smart.org.cn,liujie@smart.org.cn
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Welcome to SMART Core Facility
BD
LSRFortessa X-20
Flow Cytometry Core Facility
206, 2nd Floor, Building 1A, Weiguang Life Science Park
chenxinyi, Liujie
18718204132;13760450981
chenxinyi@smart.org.cn,liujie@smart.org.cn
1.5 lasers and 18 colors
1)355nm UltraViolet Laser
2)405nm Violet Laser
3)488nm Blue Laser
4)561nm Yellow-Green Laser
5)637nm Red Laser
2.High-Throughput sampler:96/384-well plate
1)High-throughput mode: 15 minutes per microtiter plate using 2 second acquisition
Standard mode: 44 minutes using 10 second acquisition
1. Detection of cell surface markers: cell immunophenotyping analysis, other surface protein analysis, etc.
2. Detection of intracellular proteins: cytokines, cytoskeletal molecules, cytosolic enzymes, etc.
3. Detection of intracellular ion concentration: H+, Na+, K+, Ca2+, etc.
4. Detection of nuclear components: cell cycle, total DNA analysis, etc.
5. Cell membrane: fluidity, membrane potential, membrane permeability, etc.
6. Cell function: cell survival determination, pH value, proliferation and apoptosis, drug resistance, etc.
7.Gene expression: endogenous and exogenous gene expression, fluorescent reporter gene expression, transfection efficiency, etc.