Full Spectrum High-Definition Cell Sorter Cytek Aurora CS
Cytek
Aurora
Flow Cytometry Core Facility
Building 1A, Room 206, Weiguang Life Science Park
Liu Jie
13760450981
liujie@smart.org.cn
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Welcome to SMART Core Facility
Cytek
Aurora
Flow Cytometry Core Facility
Building 1A, Room 206, Weiguang Life Science Park
Liu Jie
13760450981
liujie@smart.org.cn
1. Lasers: Five solid-state lasers with wavelengths of 355 nm, 405 nm, 488 nm, 561 nm, and 640 nm.
2. Detectors: 64 fluorescent detectors and 2 scatter detectors.
3. High-Throughput Sampler (HTS): Equipped with an ASL High-Throughput Sampler, compatible with high-throughput loading for 40-well tube racks, standard 96-well plates, and 96 deep-well plates..
4. Maximum Acquisition Rate: 35,000 cells/s.
5. Full-Spectrum Signal Detection: Detection range from 365-829 nm.
6. Fluorescence Sensitivity FITC ≤ 5 MESF, PE ≤ 4 MESF, APC ≤ 3 MESF, Pacific Blue ≤ 4.
1. Before submitting samples, it is necessary to provide the platform staff with the required experimental information and testing requirements in advance.
2. Samples must be labeled with fluorescent tags and prepared as single-cell suspensions suitable for direct measurement, with a cell density of 1–10 × 10^6 cells/ml.
3. Samples must be marked with a sample number using a marker, and for sorting experiments, clearly labeled receiving tubes must be prepared. Please use block letters for labeling.
4. During the sample submission process, please maintain low temperatures and protect samples from light.
5. For multicolor sample detection, please provide corresponding negative controls and single-stained positive controls.
1. Including but not limited to the following detection contents:
2. Cell Surface Markers
3. Extracellular Cytokine
4. Nuclear/Cytoplasmic Protein
5. Phosphorylated Protein
6. Intracellular Ion Concentration
7. DNA Content
8. Cell Membrane Status/Function
9. Cell Function Analysis
10. Gene Expression Analysis
11. Cell autofluorescence