High-speed Quantitative Imaging Cell Analyzer Cytek Imagestream MKⅡ
Cytek
ImageStream MKII
Flow Cytometry Core Facility
Building 1A, Room 206, Weiguang Life Science Park
Liu Jie
13760450981
liujie@smart.org.cn
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Welcome to SMART Core Facility
Cytek
ImageStream MKII
Flow Cytometry Core Facility
Building 1A, Room 206, Weiguang Life Science Park
Liu Jie
13760450981
liujie@smart.org.cn
1. Lasers: seven lasers with wavelengths of 375nm,405nm,488nm,561nm,592nm,642nm and 785nm;.
2. Detectors: 12 channels, including 2 Brightfield (BF) channels and 1 SSC channel.
3. objectives: 20×, 40×, and 60× magnification.
4. Image Resolution: 1 µm (20×); 0.5 µm (40×); 0.33 µm (60×).
5. Numerical Aperture: 0.5 (20× objective); 0.75 (40× objective); 0.9 (60× objective).
6. Imaging Speed: 5,000 cells/second.
7. Sample Loading Types: Compatible with 1.5ml EP tubes and 96-well plates.
1. Before submitting samples, it is necessary to provide the platform staff with the required experimental information and testing requirements in advance.
2. Samples must be labeled with fluorescent tags and prepared as single-cell suspensions suitable for direct measurement, with a cell density of 5 × 10^6 cells/ml.
3. Samples must be marked with a sample number using a marker, and for sorting experiments, clearly labeled receiving tubes must be prepared. Please use block letters for labeling.
4. During the sample submission process, please maintain low temperatures and protect samples from light.
5. For multicolor sample detection, please provide corresponding negative controls and single-stained positive controls.
Research in fields such as cell signal transduction, cell cycle, internalization and colocalization, oceanography, cell-cell interaction, cell deformation and chemotaxis, immunological synapse, intracellular and extracellular colocalization, DNA damage and repair, micronucleus counting, parasitology, stem cell biology, and microbiology.