Conventional Cell Sorter BD FACSAriaIII-Shenzhen Medical Academy of Research and Translation

Technical Parameters

Sample Submission Requirements

Application Areas

Lasers: 488 nm, 640 nm, 405 nm, 561 nm, 355 nm

Detectors: 18 fluorescent detectors and 2 scatter detectors

Maximum Acquisition Rate: 100,000 cells/s

Max Sorting Rate: 70,000 cells/s, sorting purity > 98%

Nozzles: 70 micron, 85 micron, 100 micron, 130 micron

1. Before submitting samples, it is necessary to provide the platform staff with the required experimental information and testing requirements in advance.

2. Samples must be labeled with fluorescent tags and prepared as single-cell suspensions suitable for direct measurement, with a cell density of 1–10 × 10^6 cells/ml.

3. Samples must be marked with a sample number using a marker, and for sorting experiments, clearly labeled receiving tubes must be prepared. Please use block letters for labeling.

4. During the sample submission process, please maintain low temperatures and protect samples from light.

5. For multicolor samples, please provide corresponding negative controls and single-stained positive controls.

1. Cell Analysis: Including but not limited to the following detection contents:

1) Cell Surface Markers

2) Extracellular Cytokine

3) Nuclear/Cytoplasmic Protein

4) Phosphorylated Protein

5) Intracellular Ion Concentration

6) DNA Content

7) Cell Membrane Status/Function

8) Cell Function Analysis

9) Gene Expression Analysis

2. Cell Sorting:

1) Sorting Modes: Two-way and four-way sorting; single-cell sorting; 6-384 well plate sorting

2) Index Sorting

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Conventional Cell Sorter BD FACSAriaIII

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