string(18) "en/server/670.html" Cryopreservation Services-Shenzhen Medical Academy of Research and Translation
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Cryopreservation Services

Our platform provides professional mouse sperm cryopreservation and resuscitation, as well as embryo cryopreservation and resuscitation services, with a focus on the secure preservation of valuable germplasm resources. These services significantly reduce the labor and financial costs associated with live colony maintenance, prevent the loss of strains, microbial contamination, and catastrophic losses due to natural disasters, while also lowering the cost and risk of international germplasm transport. Sperm cryopreservation is carried out using liquid nitrogen for easy operation; 30 straws are frozen per strain, ensuring a large storage capacity. Resuscitation is achieved through in vitro fertilization, enabling rapid recovery of the same strain and accommodating diverse research needs for strain preservation. For embryo cryopreservation, embryos are obtained through superovulation, natural mating, or in vitro fertilization (IVF), and preserved using the internationally recognized vitrification technique. Approximately 200 two-cell-stage embryos are frozen per strain. After resuscitation, embryos are transferred into SPF pseudo-pregnant recipient females, and SPF-grade offspring corresponding to the cryopreserved germplasm can be obtained within short period, allowing rapid re-establishment of experimental colonies.


Our platform is equipped with two 184-liter Thermo SCIENTIFIC liquid nitrogen tanks, capable of storing sperm from 1,350 strains (approximately 4,050 mice) and embryos from 202 strains. We have established four full-process technical capabilities, as listed below (Table 2.1).

 

Table 2.1 Full-Process Cryopreservation Service List

Service Item

Technical Description

Service Advantages

Deliverables

Mouse Sperm Cryopreservation

Using liquid nitrogen freezing technology, mouse sperm are standardized and stored long-term to ensure the safety of germplasm resources.

1. Frozen samples can be stored long-term (≥10 years);

2. Reduces husbandry costs and alleviates strain maintenance burden;

3. Prevents accidental colony loss;

4. Easy operation, convenient transport, and small sample volume required.

1. Frozen straws (containing sperm samples, with strain information);

2. Cryopreservation experimental data report.

Mouse Sperm Resuscitation

Frozen mouse sperm stored in liquid nitrogen are reactivated, and experimental colonies are quickly restored through in vitro fertilization and embryo transfer.

1. Sperm resuscitation rate ≥50%;

2. Efficient and fast resuscitation process for rapid colony expansion;

3. Strict quality control throughout to ensure sperm viability and genetic stability.

1. Post-resuscitation sperm viability assessment report;

2. At least three weaned positive mice delivered per strain.

Mouse Embryo Cryopreservation

Mouse embryos are vitrified and stored long-term in liquid nitrogen, enabling long-term preservation of valuable germplasm resources.

1. Suitable for preserving valuable mutants, transgenic strains, and multigenic strains;

2. Frozen samples can be stored long-term (≥10 years);

3. Over 200 embryos frozen per strain, ensuring effective preservation.

1. Cryovials (containing embryo samples, with strain information);

2. Cryopreservation experimental data report.

Mouse Embryo Resuscitation

Vitrified mouse embryos are thawed, and after viability is restored, they are transferred into surrogate females to obtain healthy offspring.

1. Gentle resuscitation process to minimize embryo damage;

2. Compatible with various valuable strains, ensuring stable embryo viability after resuscitation;

3. Controllable offspring rate to ensure strain continuity.

1. Post-resuscitation embryo viability assessment report;

2. Pregnancy monitoring report for surrogate females;

3. At least three weaned positive mice delivered per strain.

 

Our platform has provided cryopreservation services to eight research groups, delivering sperm cryopreservation for 12 strains (over 400 straws) and embryo cryopreservation for one strain (240 embryos). External services: we have completed mouse sperm cryopreservation for four strains for a research group at Southern University of Science and Technology.

 

Key technologies under recent development

Automated embryo cryopreservation: Introduction of automated equipment to optimize the embryo freezing process and reduce damage during cryopreservation. Objective: To increase the post-resuscitation embryo survival rate to nearly 100%, further improving the efficiency and stability of embryo cryopreservation and resuscitation, and better meeting the preservation needs of valuable strains.

 

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