Welcome to SMART Core Facility
Welcome to SMART Core Facility
The Biomedical Electrophysiology Core Facility supports research on bioelectrophysiological signals, including ion channel proteins, receptor proteins, neurotransmitter transporter proteins, and cell membrane pump proteins in isolated living neurons. We provide services such as drug screening, investigation of ion channel regulatory mechanisms, validation of ion channel target druggability, and cardiac safety assessment. The facility is dedicated to offering reliable and efficient one-stop electrophysiology solutions for neuroscience, cardiovascular research, and drug discovery.
The core equipment of this facility includes cellular patch clamp systems, brain slice patch clamp systems, multichannel electrical mapping systems, and microelectrode arrays (MEA), enabling cross-scale monitoring of electrical activity from single cells to tissue networks.
Equipped with multiple cellular patch clamp devices, the facility possesses comprehensive cellular patch clamp detection capabilities, offering full-process services from cell construction and electrical signal recording to data analysis. All experiments strictly adhere to standard operating procedures and rigorous quality control to ensure high-quality and reproducible data, meeting the dual standards of academic research and industrial R&D. In the field of ion channel research and drug development, the facility provides key technical support for target validation and compound screening. We support analyses such as ion channel I-V curves, activation/inactivation kinetic characteristics, use-dependent assays, and half-maximal effective concentration (EC50/IC50) evaluations, as well as fine experiments like single-channel gating kinetics recording. As of 2026, we have completed eight types of ion channel kinetic analysis services and performed over 70 ion channel drug efficacy evaluations for multiple new drug R&D projects, accelerating the development process in fields such as analgesia, arrhythmias, and neurological disorders.
In addition, the facility is deeply involved in neuroscience and induced pluripotent stem cell (iPSC) research. It enables the acquisition and analysis of electrical signals, including action potentials, ion currents, and field potentials, from a variety of sample types, such as primary neurons, brain slices, iPSC-derived neurons and cardiomyocytes, and isolated hearts. The facility has established four full-process technical capabilities, as listed below (Table 6.1).
No. | Service Item | Major Equipment Involved | Core Facility |
1 | Patch Clamp Recording - Routine Adherent Cells | Patch Clamp | Biomedical Electrophysiology Core Facility |
2 | Patch Clamp Recording - Primary and iPSC-Differentiated Cells | Patch Clamp | Biomedical Electrophysiology Core Facility |
3 | Patch Clamp Recording - Isolated Brain Slices | Slice Patch Clamp | Biomedical Electrophysiology Core Facility |
4 | High-Throughput Calcium Flux Assay | Kinetic Plate Imager | Biomedical Electrophysiology Core Facility |
To date, we have provided eight testing services for isolated mouse brain slice patch clamp recordings and four electrophysiological testing services for iPSC-differentiated neurons and cardiomyocytes for different institutions. These services have provided robust data support for disease mechanism research, drug cardiac safety evaluation, and functional analysis of neural circuits.
Master's degree graduate from Peking University with experience in patch clamp and ion channel drug screening. Primarily responsible for the operation and maintenance of instrumentations including patch clamp , kinetic plate imager and cardiac mapping.
17801061875
caiguanxing@smart.org.cn
深圳市光明区卫光生命科学园1A栋三层
硕士毕业于首都医科大学，期间从事机械敏感离子通道的脂质体/细胞电生理等相关研究工作。主要负责倒置显微镜单细胞手动膜片钳、正置显微镜脑片膜片钳、多通道电生理标测（心脏）、钙流检测工作站、细胞测钙系统等设备的日常维护、培训考核、开放共享等工作。
19022783724
ruanjiamin@smart.org.cn
深圳市光明区卫光生命科学园1A栋三层
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